continuation——and hatch at room temperature in glass containers of salt solution. describe a controlled experiment using three experiment groups that could be used to determine the best concentration of salt solution i nwhich brine shrimp will hatch.
1) a description of how the control group and each of the three experimental groups will be different
2. two conditons that must be kept constant in the control group and the experimental groups
3. data that should be collected
4. one example of experimental results that would indicate the best concentration of salt solution in which to hatch a brine shrimp egg
^^ bio hw ..i need help in the one
1. Start with 1 bottle first. Make sure that it is washed thoroughly to get rid of the smell and taste of soft drinks. You may use a diluted Clorox solution to disinfect it, but be sure to rinse off the Clorox and do not allow any Clorox to drop into your brine shrimp culture or fish tank.
2. Pour stored water into the bottle until it's about 2cm below the rim. The water should have been stored and aerated for at least a day to get rid of the chlorine. This amount of water should yield a brine shrimp culture of about 1.2 liters.
3 Put the combination hydrometer/thermometer into the water, and put about two and a half spoons full(the 5 ml size spoon) of sea salt into the water. At this stage, I use the baster to pump the solution, taking care not to spill it. You can hear the salt crystals swishing about the bottom of the bottle as it is agitated and dissolved. Check the hydrometer to see whether the mark 1.020 on it (usually marked with a green line) is level with the salt solution. If not enough, add some more salt a little at a time, and mix again with the baster until the measurement is correct. If you do not have a baster, you can use your air tube to aerate the solution, but this method will take a longer time, and can get quite messy, as the bubbles from the strong aeration burst and you may find a white coating of salt and brine on your nearby tank, etc.
4. After the salt solution is of required salinity, remove both the air tube and hydrometer from the culture bottle, and put them temporarily into the fish tank. Then measure and put 1/2 or slightly more than half spoon (the 2.5 ml spoon) of brine shrimp eggs into the solution. The reason for taking out the hydrometer and air-tube temporarily is that brine shrimp eggs are very light, and will stick to objects as well as to the side of the bottle if the water is agitated while the brine shrimp eggs are poured into it. By putting the eggs into a completely still solution for about half to one hour, a lot of the eggs will become wet by themselves, and sink into the solution. This will give a higher yield of brine shrimps. After 1/2 an hour or so, put back the hydrometer and air-tube into the brine shrimp solution, and turn on the air to bubble slowly. Most of the eggs will begin to sink into the water.
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